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cd31  (R&D Systems)


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    Structured Review

    R&D Systems cd31
    ID tissue exhibits higher densities of fibroblasts, macrophages, and microvessels compared to SC tissue. Immunofluorescence analysis comparing the expression of Vimentin (a fibroblast marker), CD68 (a macrophage marker), and <t>CD31</t> (an endothelial cell marker) in ID and SC tissues. For each group, n = 3; data represent mean ± s.d.; ∗P < 0.05 and ∗∗P < 0.01.
    Cd31, supplied by R&D Systems, used in various techniques. Bioz Stars score: 98/100, based on 1263 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rat+anti+mouse+antibody/Human%2FMouse%2FRat+CD31%2FPECAM-1+Antibody/pmc13091142-299-20-21
    Average 98 stars, based on 1263 article reviews
    cd31 - by Bioz Stars, 2026-09
    98/100 stars

    Images

    1) Product Images from "Injection site dictates the immune response to a biodegradable polymer and corresponding collagen regeneration"

    Article Title: Injection site dictates the immune response to a biodegradable polymer and corresponding collagen regeneration

    Journal: Bioactive Materials

    doi: 10.1016/j.bioactmat.2026.04.004

    ID tissue exhibits higher densities of fibroblasts, macrophages, and microvessels compared to SC tissue. Immunofluorescence analysis comparing the expression of Vimentin (a fibroblast marker), CD68 (a macrophage marker), and CD31 (an endothelial cell marker) in ID and SC tissues. For each group, n = 3; data represent mean ± s.d.; ∗P < 0.05 and ∗∗P < 0.01.
    Figure Legend Snippet: ID tissue exhibits higher densities of fibroblasts, macrophages, and microvessels compared to SC tissue. Immunofluorescence analysis comparing the expression of Vimentin (a fibroblast marker), CD68 (a macrophage marker), and CD31 (an endothelial cell marker) in ID and SC tissues. For each group, n = 3; data represent mean ± s.d.; ∗P < 0.05 and ∗∗P < 0.01.

    Techniques Used: Immunofluorescence, Expressing, Marker

    Related Articles

    Saline:

    Article Title: Interleukin-1β blockade improves cardiac remodeling after myocardial infarction without interrupting the inflammasome in the mouse
    Article Snippet: .. Following saturation with 5% milk in phosphate buffered saline the membrane was incubated with a rat anti-mouse antibody raised against CD45 (R&D systems, Minneapolis, MN). .. To normalize the protein loading a monoclonal antibody for β-actin (Sigma Aldrich) was used.

    Article Title: GS-6201, a Selective Blocker of the A 2B Adenosine Receptor, Attenuates Cardiac Remodeling after Acute Myocardial Infarction in the Mouse
    Article Snippet: .. After saturation with 5% milk in phosphate-buffered saline the membrane was incubated with a rat anti-mouse antibody raised against CD45 (R&D Systems, Minneapolis, MN). .. To normalize the protein loading a monoclonal antibody for β-actin (Sigma-Aldrich) was used.

    Membrane:

    Article Title: Interleukin-1β blockade improves cardiac remodeling after myocardial infarction without interrupting the inflammasome in the mouse
    Article Snippet: .. Following saturation with 5% milk in phosphate buffered saline the membrane was incubated with a rat anti-mouse antibody raised against CD45 (R&D systems, Minneapolis, MN). .. To normalize the protein loading a monoclonal antibody for β-actin (Sigma Aldrich) was used.

    Article Title: GS-6201, a Selective Blocker of the A 2B Adenosine Receptor, Attenuates Cardiac Remodeling after Acute Myocardial Infarction in the Mouse
    Article Snippet: .. After saturation with 5% milk in phosphate-buffered saline the membrane was incubated with a rat anti-mouse antibody raised against CD45 (R&D Systems, Minneapolis, MN). .. To normalize the protein loading a monoclonal antibody for β-actin (Sigma-Aldrich) was used.

    Article Title: Interleukin-1β blockade improves cardiac remodelling after myocardial infarction without interrupting the inflammasome in the mouse.
    Article Snippet: .. Following saturation with 5% milk in PBS, the membrane was incubated with a rat anti-mouse antibody raised against CD45 (R&D Systems, Minneapolis, MN, USA). .. To normalize the protein loading, a monoclonal antibody for β-actin (Sigma Aldrich) was used.

    Incubation:

    Article Title: Interleukin-1β blockade improves cardiac remodeling after myocardial infarction without interrupting the inflammasome in the mouse
    Article Snippet: .. Following saturation with 5% milk in phosphate buffered saline the membrane was incubated with a rat anti-mouse antibody raised against CD45 (R&D systems, Minneapolis, MN). .. To normalize the protein loading a monoclonal antibody for β-actin (Sigma Aldrich) was used.

    Article Title: GS-6201, a Selective Blocker of the A 2B Adenosine Receptor, Attenuates Cardiac Remodeling after Acute Myocardial Infarction in the Mouse
    Article Snippet: .. After saturation with 5% milk in phosphate-buffered saline the membrane was incubated with a rat anti-mouse antibody raised against CD45 (R&D Systems, Minneapolis, MN). .. To normalize the protein loading a monoclonal antibody for β-actin (Sigma-Aldrich) was used.

    Article Title: Interleukin-1β blockade improves cardiac remodelling after myocardial infarction without interrupting the inflammasome in the mouse.
    Article Snippet: .. Following saturation with 5% milk in PBS, the membrane was incubated with a rat anti-mouse antibody raised against CD45 (R&D Systems, Minneapolis, MN, USA). .. To normalize the protein loading, a monoclonal antibody for β-actin (Sigma Aldrich) was used.

    Transfection:

    Article Title: Paralysis of the cytotoxic granule machinery is a new cancer immune evasion mechanism mediated by chitinase 3-like-1.
    Article Snippet: Absorbance was measured at 450 nm using Clariostar Plate reader (BMG Labtech). .. The endogenous human CHI3L1 secreted from HCC1569 cells or the murine CHI3L1 secreted by transfected JIMT- 1 cells were detected by western blot analysis of supernatant from 70% confluent culture (20 μL) using goat antihuman CHI3L1 polyclonal antibody (R&D systems) and rat anti- mouse antibody (R&D systems), respectively. .. The CyQUANT Cell Proliferation Assay (Thermofisher Scientific) was used to determine the effect of CHI3L1 treatment or overexpression on the proliferation of tumor cells in vitro before conducting tumor experiments with these cells.

    Article Title: Paralysis of the cytotoxic granule machinery is a new cancer immune evasion mechanism mediated by chitinase 3-like-1
    Article Snippet: Absorbance was measured at 450 nm using Clariostar Plate reader (BMG Labtech). .. The endogenous human CHI3L1 secreted from HCC1569 cells or the murine CHI3L1 secreted by transfected JIMT-1 cells were detected by western blot analysis of supernatant from 70% confluent culture (20 μL) using goat anti-human CHI3L1 polyclonal antibody (R&D systems) and rat anti-mouse antibody (R&D systems), respectively. .. The CyQUANT Cell Proliferation Assay (Thermofisher Scientific) was used to determine the effect of CHI3L1 treatment or overexpression on the proliferation of tumor cells in vitro before conducting tumor experiments with these cells.

    Western Blot:

    Article Title: Paralysis of the cytotoxic granule machinery is a new cancer immune evasion mechanism mediated by chitinase 3-like-1.
    Article Snippet: Absorbance was measured at 450 nm using Clariostar Plate reader (BMG Labtech). .. The endogenous human CHI3L1 secreted from HCC1569 cells or the murine CHI3L1 secreted by transfected JIMT- 1 cells were detected by western blot analysis of supernatant from 70% confluent culture (20 μL) using goat antihuman CHI3L1 polyclonal antibody (R&D systems) and rat anti- mouse antibody (R&D systems), respectively. .. The CyQUANT Cell Proliferation Assay (Thermofisher Scientific) was used to determine the effect of CHI3L1 treatment or overexpression on the proliferation of tumor cells in vitro before conducting tumor experiments with these cells.

    Article Title: Paralysis of the cytotoxic granule machinery is a new cancer immune evasion mechanism mediated by chitinase 3-like-1
    Article Snippet: Absorbance was measured at 450 nm using Clariostar Plate reader (BMG Labtech). .. The endogenous human CHI3L1 secreted from HCC1569 cells or the murine CHI3L1 secreted by transfected JIMT-1 cells were detected by western blot analysis of supernatant from 70% confluent culture (20 μL) using goat anti-human CHI3L1 polyclonal antibody (R&D systems) and rat anti-mouse antibody (R&D systems), respectively. .. The CyQUANT Cell Proliferation Assay (Thermofisher Scientific) was used to determine the effect of CHI3L1 treatment or overexpression on the proliferation of tumor cells in vitro before conducting tumor experiments with these cells.



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    Image Search Results


    BAITs promote DC activation and antigen presentation via enhanced lysosomal processing. (A) Schematic for MHC Ⅰ/Ⅱ immunostaining. DCs were treated with antigens or BAITs (12 h), then incubated in fresh medium (with TGFβ) for 24 h before staining. (B, C) Quantification of surface MHC Ⅰ (B) and MHC Ⅱ (C) on DCs (n = 8; n.s. is P > 0.05, ∗∗∗ is P < 0.001, ∗∗∗∗ is P < 0.0001 by one-way ANOVA with Bonferroni post-hoc test). (D, E) Representative immunofluorescence images of DCs (blue: nuclei; yellow: LAMP1; green: MHC Ⅰ; magenta: MHC Ⅱ). BAIT-treated DCs show strong surface MHC and minimal intracellular antigen signal, whereas antigen-treated DCs show retained antigen (red) and weak surface MHC signal. (F–H) Flow cytometric analysis of MHC Ⅰ (F), MHC Ⅱ (G), and CD80 (H) expression on murine CD11c + DCs after exposure to antigens or BAITs. (I) Quantification of MHC Ⅰ, MHC Ⅱ, and CD80 expression in DCs by flow cytometry (n = 3; ∗∗∗ is P < 0.001, ∗∗∗∗ is P < 0.0001 by two-way ANOVA with Bonferroni post-hoc test). (J) Heatmap of DC activation-related gene expression after treatment with antigens or BAITs, highlighting upregulation of maturation and antigen presentation genes and downregulation of immunosuppressive genes by BAITs (n = 3). Data are presented as mean ± SD.

    Journal: Bioactive Materials

    Article Title: Countering postoperative immune suppression with a self-assembling dendritic cell nanovaccine

    doi: 10.1016/j.bioactmat.2026.05.005

    Figure Lengend Snippet: BAITs promote DC activation and antigen presentation via enhanced lysosomal processing. (A) Schematic for MHC Ⅰ/Ⅱ immunostaining. DCs were treated with antigens or BAITs (12 h), then incubated in fresh medium (with TGFβ) for 24 h before staining. (B, C) Quantification of surface MHC Ⅰ (B) and MHC Ⅱ (C) on DCs (n = 8; n.s. is P > 0.05, ∗∗∗ is P < 0.001, ∗∗∗∗ is P < 0.0001 by one-way ANOVA with Bonferroni post-hoc test). (D, E) Representative immunofluorescence images of DCs (blue: nuclei; yellow: LAMP1; green: MHC Ⅰ; magenta: MHC Ⅱ). BAIT-treated DCs show strong surface MHC and minimal intracellular antigen signal, whereas antigen-treated DCs show retained antigen (red) and weak surface MHC signal. (F–H) Flow cytometric analysis of MHC Ⅰ (F), MHC Ⅱ (G), and CD80 (H) expression on murine CD11c + DCs after exposure to antigens or BAITs. (I) Quantification of MHC Ⅰ, MHC Ⅱ, and CD80 expression in DCs by flow cytometry (n = 3; ∗∗∗ is P < 0.001, ∗∗∗∗ is P < 0.0001 by two-way ANOVA with Bonferroni post-hoc test). (J) Heatmap of DC activation-related gene expression after treatment with antigens or BAITs, highlighting upregulation of maturation and antigen presentation genes and downregulation of immunosuppressive genes by BAITs (n = 3). Data are presented as mean ± SD.

    Article Snippet: After treatment, the cells were washed twice with PBS and fixed in 4% paraformaldehyde for 20 min. For intracellular protein staining, fixed cells were permeabilized with 0.1% Triton X-100 at room temperature for 10 min. Next, the cells were blocked with 2% BSA and incubated with rat CoraLite Plus 488 anti-mouse LAMP1 antibody (1:200) and rabbit anti-mouse pSAP (1:200) antibody overnight at 4 °C.

    Techniques: Activation Assay, Immunopeptidomics, Immunostaining, Incubation, Staining, Immunofluorescence, Expressing, Flow Cytometry, Gene Expression

    ID tissue exhibits higher densities of fibroblasts, macrophages, and microvessels compared to SC tissue. Immunofluorescence analysis comparing the expression of Vimentin (a fibroblast marker), CD68 (a macrophage marker), and CD31 (an endothelial cell marker) in ID and SC tissues. For each group, n = 3; data represent mean ± s.d.; ∗P < 0.05 and ∗∗P < 0.01.

    Journal: Bioactive Materials

    Article Title: Injection site dictates the immune response to a biodegradable polymer and corresponding collagen regeneration

    doi: 10.1016/j.bioactmat.2026.04.004

    Figure Lengend Snippet: ID tissue exhibits higher densities of fibroblasts, macrophages, and microvessels compared to SC tissue. Immunofluorescence analysis comparing the expression of Vimentin (a fibroblast marker), CD68 (a macrophage marker), and CD31 (an endothelial cell marker) in ID and SC tissues. For each group, n = 3; data represent mean ± s.d.; ∗P < 0.05 and ∗∗P < 0.01.

    Article Snippet: After antigen retrieval and blocking, sections were incubated overnight at 4 °C with primary antibodies targeting vimentin, CD68 (ABclonal, A20803), CD31 (R&D SYSTEMS, AF3628), HMGB1 (Cell Signaling Technology, 3935), HSP70 (ABclonal, A23457), NF-κB p65 (ABclonal, A19653), CD206 (Cell Signaling Technology, 24595), and FAPα (ABclonal, A23789 ).

    Techniques: Immunofluorescence, Expressing, Marker